7VZT

A human neutralizing antibody targeting SARS-CoV-2 RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	150.605, 150.605, 121.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.300 - 3.410
R-factor	0.222
Rwork	0.217
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lzg 4tsa
RMSD bond length	0.005
RMSD bond angle	1.189
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	49.400
High resolution limit [Å]	3.400	3.410
Number of reflections	15881	15881
<I/σ(I)>	12.6
Completeness [%]	71.9
Redundancy	34.7
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100mM HEPES (pH 7.5), 40% (v/v) Polyethylene glycol monomethyl ether 400