7VZS
FAD-dpendent Glucose Dehydrogenase complexed with an inhibitor at pH7.56
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.933, 67.079, 150.681 |
| Unit cell angles | 90.00, 97.13, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.15425 |
| Rwork | 0.152 |
| R-free | 0.19033 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.088 | 0.195 |
| Number of reflections | 92885 | 9552 |
| <I/σ(I)> | 15.4 | 2.4 |
| Completeness [%] | 95.3 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.56 | 293 | PEG 10000, HEPES Na buffer, soaking a crystal in a crystallization solution containing 1 M D-glucal |
