7VZA
The crystal structure of Non-hydrolyzing UDPGlcNAc 2-epimerase in complex with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-04-27 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 81.820, 104.770, 106.314 |
| Unit cell angles | 74.54, 73.05, 68.39 |
Refinement procedure
| Resolution | 19.895 - 2.580 |
| Rwork | 0.210 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vgv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.454 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (v1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.895 | 2.680 |
| High resolution limit [Å] | 2.580 | 2.600 |
| Rmeas | 0.050 | 0.050 |
| Number of reflections | 91395 | 91395 |
| <I/σ(I)> | 23.8 | |
| Completeness [%] | 94.1 | |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M lithium sulfate, 0.1M Bis tris pH5.5, 25% PEG 3350 |
