7VZ6

The crystal structure of Non-hydrolyzing UDPGlcNAc 2-epimerase in complex with UDP-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-12-22
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	82.833, 104.941, 106.840
Unit cell angles	74.87, 72.92, 68.08

Refinement procedure

Resolution	21.558 - 2.092
Rwork	0.201
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vgv
RMSD bond length	0.008
RMSD bond angle	1.495
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.560	2.180
High resolution limit [Å]	2.090	2.100
Rmeas	0.057	0.057
Number of reflections	176474	176474
<I/σ(I)>	19.7
Completeness [%]	95.2
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2M lithium sulfate, 0.1M Bis tris pH 5.5, 25% PEG 3350