7VZ6
The crystal structure of Non-hydrolyzing UDPGlcNAc 2-epimerase in complex with UDP-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-22 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.833, 104.941, 106.840 |
| Unit cell angles | 74.87, 72.92, 68.08 |
Refinement procedure
| Resolution | 21.558 - 2.092 |
| Rwork | 0.201 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vgv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.495 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.560 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.100 |
| Rmeas | 0.057 | 0.057 |
| Number of reflections | 176474 | 176474 |
| <I/σ(I)> | 19.7 | |
| Completeness [%] | 95.2 | |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2M lithium sulfate, 0.1M Bis tris pH 5.5, 25% PEG 3350 |
