7VZ2

Crystal structure of chromodomain of Arabidopsis LHP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-23
Detector	AGILENT ATLAS CCD
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	31.708, 31.708, 106.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.480 - 1.700
R-factor	0.2493
Rwork	0.245
R-free	0.29120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q3l
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.480	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	7107	379
<I/σ(I)>	47.9
Completeness [%]	97.3
Redundancy	20
CC(1/2)	1.000	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20 mM Tris, pH 7.5, 150 mM NaCl and 1 mM DTT