7VYY
The crystal structure of Non-hydrolyzing UDPGlcNAc 2-epimerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-07-24 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.349, 106.052, 94.343 |
| Unit cell angles | 90.00, 124.87, 90.00 |
Refinement procedure
| Resolution | 25.449 - 2.443 |
| Rwork | 0.228 |
| R-free | 0.27220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vgv |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.313 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.450 | 2.550 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmeas | 0.055 | 0.055 |
| Number of reflections | 28957 | 27447 |
| <I/σ(I)> | 26.6 | |
| Completeness [%] | 85.9 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M Potassium/Sodium tartrate, 0.1 M Bis Tris-propane pH 7.5, 20% PEG 3350 |
