7VYX

Crystal structure of the selenomethionine(SeMet)-derived Cas12c1 (D969A) ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-12-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	93.127, 149.850, 173.246
Unit cell angles	90.00, 91.59, 90.00

Refinement procedure

Resolution	49.730 - 3.200
R-factor	0.2575
Rwork	0.256
R-free	0.29240
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.13-2998)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.130	1.634
Number of reflections	78406	7804
<I/σ(I)>	14.8	1.18
Completeness [%]	99.9	100
Redundancy	6.8	7
CC(1/2)	0.996	0.493

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Tris-HCl (pH 8.2), 16% polyethylene glycol 3350 (w/v), 0.02 M citric acid, 0.05 M lithium acetate dihydrate