7VYW

Crystal structure of the chromodomain of Arabidopsis LHP1 in complex with methylated histone H3K9 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	26.088, 28.547, 70.519
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.260 - 1.600
R-factor	0.1843
Rwork	0.182
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q3l
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.260	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	6673	294
<I/σ(I)>	23.2
Completeness [%]	90.3
Redundancy	4.6
CC(1/2)	0.982	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Hepes, pH 7.5, 2.0 M Ammonium sulfate