7VXT
Crystal structure of a selenomethionine-labeled BPSL1038 from Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97972 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.726, 115.891, 46.812 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.970 - 1.880 |
| R-factor | 0.1493 |
| Rwork | 0.148 |
| R-free | 0.17830 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.155 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.970 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmeas | 0.104 | 0.720 |
| Rpim | 0.041 | 0.312 |
| Number of reflections | 19376 | 1140 |
| <I/σ(I)> | 13.4 | 2.4 |
| Completeness [%] | 99.6 | 94.6 |
| Redundancy | 6.4 | 5 |
| CC(1/2) | 0.997 | 0.826 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M sodium acetate pH 4.6, 2 M sodium formate |
