7VX0
The structure of GdmN complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 111.717, 111.717, 230.717 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.640 - 2.000 |
R-factor | 0.1837 |
Rwork | 0.182 |
R-free | 0.21150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ven |
RMSD bond length | 0.007 |
RMSD bond angle | 0.898 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.104 | 1.025 |
Number of reflections | 113024 | 5618 |
<I/σ(I)> | 36.368 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M Lithium sulfate monohydrate, 25% w/v Polyethylene glycol 3,350 and 0.1 M Tris, pH 8.5 |