7VWP
Structure of the flavin-dependent monooxygenase FlsO1 from the biosynthesis of fluostatinsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-06 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.540, 84.636, 102.799 |
| Unit cell angles | 91.31, 107.86, 115.39 |
Refinement procedure
| Resolution | 13.182 - 2.300 |
| Rwork | 0.211 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qa1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrysalisPro (1.171.39.7e) |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.182 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.066 | 0.390 |
| Number of reflections | 95967 | 4703 |
| <I/σ(I)> | 12.9 | 2.8 |
| Completeness [%] | 98.9 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.02 M Sodium/potassium phosphate, 0.1 M Bis-Tris propane, pH 6.5, 20 % w/v PEG 3350 |
