7VWA
Helicoverpa armigera pheromone-binding protein PBP1 with Z-9-hexadecenal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.907, 33.356, 56.044 |
| Unit cell angles | 90.00, 98.79, 90.00 |
Refinement procedure
| Resolution | 32.540 - 2.100 |
| R-factor | 0.20066 |
| Rwork | 0.199 |
| R-free | 0.23592 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vw9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.515 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.057 | 0.302 |
| Number of reflections | 7259 | 277 |
| <I/σ(I)> | 31.1 | |
| Completeness [%] | 98.2 | 81 |
| Redundancy | 4.9 | 3.7 |
| CC(1/2) | 0.991 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | peg |
