7VW9

Helicoverpa armigera pheromone-binding protein PBP1 at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	32.431, 33.376, 55.380
Unit cell angles	90.00, 99.66, 90.00

Refinement procedure

Resolution	50.000 - 2.050
R-factor	0.2239
Rwork	0.222
R-free	0.26546
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vw8
RMSD bond length	0.007
RMSD bond angle	0.994
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.050
Number of reflections	7557	344
<I/σ(I)>	41.2
Completeness [%]	95.6
Redundancy	3.5
CC(1/2)	0.992	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	peg