7VVB

Crystal Structure of KRas4A(GMPPNP-bound) in complex with the Ras-binding domain(RBD) of SIN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 61 2 2
Unit cell lengths	101.660, 101.660, 124.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.380 - 1.700
R-factor	0.1739
Rwork	0.173
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g0n
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.380	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.068	0.596
Number of reflections	79142	4143
<I/σ(I)>	26.68
Completeness [%]	99.8	99.98
Redundancy	18.6	15.8
CC(1/2)	0.999	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	278	PEG 5000 MME