7VV9

Crystal Structure of HRas(GMPPNP-bound) in complex with the Ras-binding domain(RBD) of SIN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 61 2 2
Unit cell lengths	101.238, 101.238, 124.371
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.120 - 1.600
R-factor	0.1825
Rwork	0.181
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g0n
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.120	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.060	0.816
Number of reflections	50105	4914
<I/σ(I)>	28.52
Completeness [%]	99.9
Redundancy	19.5
CC(1/2)	1.000	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	278	PEG 20000, Sodium citrate