Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2017-12-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 31 |
| Unit cell lengths | 60.277, 60.277, 131.385 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.801 - 2.319 |
| R-factor | 0.1985 |
| Rwork | 0.196 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yuo |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.319 | 2.319 |
| Rmerge | 0.064 | 0.509 |
| Number of reflections | 23123 | 2309 |
| <I/σ(I)> | 18.1 | 2.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 25% PEG 4000, 100mM MES Sodium Salt pH 6.5, 200mM Magnesium Chloride |
