7VUC
Myoglobin mutant L29I/H64G/V68A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2021-01-05 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.019, 90.019, 45.368 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.680 - 1.400 |
| R-factor | 0.1774 |
| Rwork | 0.176 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mlu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.772 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.680 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rpim | 0.027 | 0.433 |
| Number of reflections | 41415 | 1982 |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.998 | 0.488 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM Tris pH 8, 2.4M ammonium sulphate |
