7VU9

Pholiota squarrosa lectin (PhoSL) in complex with fucose(alpha1-6)[GlcNAc(beta1-4)]GlcNAc

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 2 2 2
Unit cell lengths	126.739, 127.057, 39.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.865 - 2.154
Rwork	0.179
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a86
RMSD bond length	0.010
RMSD bond angle	1.774
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.870	2.280
High resolution limit [Å]	2.154	2.154
Rmerge	0.087	0.661
Number of reflections	17730	5358
<I/σ(I)>	9.18	1.81
Completeness [%]	99.5	99.3
Redundancy	3.5	3.43
CC(1/2)	0.996	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	4.3 M sodium formate (pH 7.0)