7VU1
Chitoporin from Escherichia coli complex with chitohexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2016-09-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97883 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.737, 58.551, 133.059 |
| Unit cell angles | 90.00, 110.71, 90.00 |
Refinement procedure
| Resolution | 62.340 - 1.900 |
| R-factor | 0.2109 |
| Rwork | 0.209 |
| R-free | 0.24820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vtz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.250 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.500 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 107599 | 5303 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.974 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30-35% PEG 400, 0.1 M Hepes pH 7.5 |
