7VTL

Crystal structure of GH family 64 beta-1,3-glucanase complexed with Laminaritriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-01
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	81.680, 81.680, 161.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.377 - 2.070
R-factor	0.208790400183
Rwork	0.208
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vtk
RMSD bond length	0.008
RMSD bond angle	1.040
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2474)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.380	2.122
High resolution limit [Å]	2.070	2.070
Rmerge	0.080	0.079
Number of reflections	38667	38667
<I/σ(I)>	21.6
Completeness [%]	97.0
Redundancy	18.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.1M sodium malonate pH 7.0, 0.1M HEPES pH 7.0