7VT9

CRYSTAL STRUCTURE AT 3.4 ANGSTROMS RESOLUTION OF Maltodextrin glucosidase, MalZ, FROM Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	195
Detector technology	CCD
Collection date	2013-10-02
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	I 41 2 2
Unit cell lengths	204.186, 204.186, 267.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.910 - 3.300
R-factor	0.3051
Rwork	0.303
R-free	0.34310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d0g
RMSD bond length	0.015
RMSD bond angle	1.829
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.910	3.418
High resolution limit [Å]	3.300	3.300
Rmerge	0.355	0.001
Number of reflections	43678	4169
<I/σ(I)>	4.02
Completeness [%]	99.9	99.2
Redundancy	25.4	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	20% polyacrylic acid 5100, 100 mM HEPES/Sodium hydroxide pH 7.0 and 20 mM Magnesium chloride