7VT6
Crystal structure of CBM deleted MtGlu5 in complex with BGC.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-08-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.683, 75.235, 83.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.280 - 1.530 |
R-factor | 0.1498 |
Rwork | 0.148 |
R-free | 0.17510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mmu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.107 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.053 | 0.186 |
Rmeas | 0.062 | 0.210 |
Rpim | 0.031 | 0.097 |
Number of reflections | 45521 | 4725 |
<I/σ(I)> | 33.82 | |
Completeness [%] | 94.8 | |
Redundancy | 4.2 | |
CC(1/2) | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 20 mM Tris, 100 mM NaCl, pH 8.0 |