7VT1
Acyltransferase from the 9th Module of Salinomycin Polyketide Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97778 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.175, 102.565, 134.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.950 - 2.500 |
| R-factor | 0.2139 |
| Rwork | 0.212 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6iyt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.326 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.086 | 0.841 |
| Number of reflections | 32005 | 1554 |
| <I/σ(I)> | 22.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.15 M DL-Malic acid (pH 7.0), 20% (v/v) PEG 3350 |
