7VSK
Crystal structure of E.coli pseudouridine kinase PsuK complexed with pseudouridine.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-23 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 182.634, 182.634, 51.228 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.410 - 2.310 |
R-factor | 0.2303 |
Rwork | 0.228 |
R-free | 0.27550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vkp |
RMSD bond length | 0.008 |
RMSD bond angle | 0.988 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.137 | 0.454 |
Rmeas | 0.141 | 0.483 |
Rpim | 0.029 | 0.158 |
Number of reflections | 22581 | 2166 |
<I/σ(I)> | 13.67 | 3 |
Completeness [%] | 99.5 | 98.2 |
Redundancy | 17.7 | 8.3 |
CC(1/2) | 0.995 | 0.130 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% (w/v) Polyacrylic acid 5100, 0.1M HEPES/Sodium hydroxide pH 7.0, 0.02M Magnesium chloride |