7VSE

E. coli Ribonuclease HI in complex one Zn2+ (His124 N-epsilon binding)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	95
Detector technology	PIXEL
Collection date	2021-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 2 2 21
Unit cell lengths	60.650, 64.586, 79.104
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.130 - 2.080
R-factor	0.2321
Rwork	0.230
R-free	0.27630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z0u
RMSD bond length	0.003
RMSD bond angle	0.597
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.130	2.140
High resolution limit [Å]	2.080	2.080
Rmerge	0.073	0.354
Rmeas	0.086	0.415
Rpim	0.046	0.215
Number of reflections	35934	1463
<I/σ(I)>	13.6
Completeness [%]	99.9	99.9
Redundancy	6.5
CC(1/2)	0.997	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.2 M ammonium sulfate, 0.1 M sodium acetate trihydrate pH 4.6, 30% w/v polyethylene glycol monomethyl ether 2,000