7VSC

E. coli Ribonuclease HI in complex with one Mg2+ (1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	95
Detector technology	PIXEL
Collection date	2021-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 43 2 2
Unit cell lengths	61.998, 61.998, 80.138
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.040 - 1.830
R-factor	0.2052
Rwork	0.203
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g15
RMSD bond length	0.004
RMSD bond angle	0.629
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.040	1.870
High resolution limit [Å]	1.830	1.830
Rmerge	0.133	1.417
Rmeas	0.144	1.533
Rpim	0.056	0.580
Number of reflections	14230	844
<I/σ(I)>	11.2
Completeness [%]	99.2
Redundancy	12.4
CC(1/2)	0.993	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M Sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate pH 6.5, 30% w/v polyethylene glycol 8,000