7VSA

E. coli Ribonuclease HI in complex with two Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	95
Detector technology	PIXEL
Collection date	2020-10-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 2 2 21
Unit cell lengths	61.639, 64.288, 80.288
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.890 - 1.760
R-factor	0.202
Rwork	0.201
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z0u
RMSD bond length	0.006
RMSD bond angle	0.804
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.890	1.790
High resolution limit [Å]	1.760	1.760
Rmerge	0.058	0.596
Rmeas	0.070	0.706
Rpim	0.038	0.381
Number of reflections	32256	1799
<I/σ(I)>	14.9
Completeness [%]	99.8
Redundancy	6.3
CC(1/2)	0.997	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M ammonium sulfate, 0.1 M MES monohydrate pH 6.5, 30% w/v polyethylene glycol monomethyl ether 5,000