7VS6
Phloem lectin (PP2) structure -native form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 52.690, 52.690, 261.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.500 - 2.500 |
| Rwork | 0.196 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.740 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.198 |
| Number of reflections | 575252 | 8209 |
| <I/σ(I)> | 56.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 70.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 1.0 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6 |
