7VS3
The crystal structure of rat calcium-dependent activator protein for secretion (CAPS) C2PH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.940, 62.452, 64.071 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.561 - 2.595 |
| R-factor | 0.2261 |
| Rwork | 0.223 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wi1 3w56 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.613 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmeas | 0.225 | 0.779 |
| Number of reflections | 7469 | 716 |
| <I/σ(I)> | 11.1 | 3 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 9 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293.15 | 0.1M Tris pH 8.5, 2.5M ammonium sulfate |
