7VRB
Structure of the Human BRG1/SS18 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9788 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 101.607, 105.829, 225.857 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.232 - 2.389 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.25040 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.885 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.232 | 2.430 |
| High resolution limit [Å] | 2.389 | 2.390 |
| Rpim | 0.018 | 0.297 |
| Number of reflections | 24275 | 1205 |
| <I/σ(I)> | 39.16 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.16 M sodium phosphate monobasic monohydrate and 0.24 M potassium phosphate dibasic |
