7VQU

Crystal structure of LSD1 in complex with compound S1427

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	188.248, 188.248, 106.997
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.450 - 2.940
R-factor	0.2212
Rwork	0.219
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kgp
RMSD bond length	0.010
RMSD bond angle	1.340
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.450	3.120
High resolution limit [Å]	2.940	2.940
Rmeas	0.116	2.358
Rpim	0.026	0.515
Number of reflections	24298	3843
<I/σ(I)>	22.2	1.9
Completeness [%]	100.0
Redundancy	19.8
CC(1/2)	1.000	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M MES (pH 6.2-6.3), 0.2M diammonium tartrate, 0.0005M TCEP, 10-12% PEG 3350