7VQK
Catalytic manifolds of a FMN-dependent oxidoreductase RubE7, expanding the functional diversity of the flavoenzyme superfamily
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.701, 54.410, 69.621 |
Unit cell angles | 90.00, 97.37, 90.00 |
Refinement procedure
Resolution | 48.100 - 2.000 |
R-factor | 0.1692 |
Rwork | 0.166 |
R-free | 0.21070 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.806 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.100 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.066 | 0.181 |
Number of reflections | 56548 | 15145 |
<I/σ(I)> | 18.8 | 8.8 |
Completeness [%] | 95.0 | 95.7 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 0.1 M Imidazole, 5% PEG3350, 5% Tacsimate pH 7.0, 5% 2-propanol |