7VQK
Catalytic manifolds of a FMN-dependent oxidoreductase RubE7, expanding the functional diversity of the flavoenzyme superfamily
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.701, 54.410, 69.621 |
| Unit cell angles | 90.00, 97.37, 90.00 |
Refinement procedure
| Resolution | 48.100 - 2.000 |
| R-factor | 0.1692 |
| Rwork | 0.166 |
| R-free | 0.21070 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.806 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.181 |
| Number of reflections | 56548 | 15145 |
| <I/σ(I)> | 18.8 | 8.8 |
| Completeness [%] | 95.0 | 95.7 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 0.1 M Imidazole, 5% PEG3350, 5% Tacsimate pH 7.0, 5% 2-propanol |
