7VQD
Structure of MA1831 from Methanosarcina acetivorans in complex with farnesyl pyrophosphate and geranylgeranyl pyrophosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-28 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34138 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.061, 81.581, 59.755 |
| Unit cell angles | 90.00, 102.89, 90.00 |
Refinement procedure
| Resolution | 33.430 - 2.390 |
| R-factor | 0.175 |
| Rwork | 0.171 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7caq |
| Data reduction software | SAINT |
| Data scaling software | Aimless (0.6.3) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.430 | 2.490 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.135 | 0.855 |
| Number of reflections | 20642 | 2369 |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 91.4 | 43.6 |
| Redundancy | 3.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 1.5M ammonium sulfate, 0.1M Tris (pH 8.0), 12% glycerol |
