7VPR
Crystal structure of the ligand-binding domain of C. glabrata Upc2 in complex with ergosterol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-11-12 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.423, 105.626, 98.872 |
| Unit cell angles | 90.00, 93.28, 90.00 |
Refinement procedure
| Resolution | 27.600 - 2.590 |
| R-factor | 0.2315 |
| Rwork | 0.231 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n9n |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.914 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.590 | 2.600 |
| Rmerge | 0.073 | 0.391 |
| Number of reflections | 34631 | 1404 |
| <I/σ(I)> | 23.7 | |
| Completeness [%] | 97.8 | 79.5 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 295 | 0.1 M Na-Acetate pH 4.0, 10% PEG 4000, 0.1 M Sodium Citrate |
