7VP8

Crystal structure of ferritin from Ureaplasma urealyticum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	F 4 3 2
Unit cell lengths	178.441, 178.441, 178.441
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.341 - 2.002
R-factor	0.1623
Rwork	0.159
R-free	0.19050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold2
Data reduction software	HKL-2000
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.341	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.151	0.630
Number of reflections	16992	832
<I/σ(I)>	42.6	9.37
Completeness [%]	100.0
Redundancy	37.9
CC(1/2)	0.995	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	NaCl, PEG 6000