7VP7

Structure of a transcription factor and DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	78.756, 78.756, 82.803
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.562 - 2.653
Rwork	0.206
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vp2
RMSD bond length	0.010
RMSD bond angle	1.061
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.562	2.748
High resolution limit [Å]	2.653	2.653
Rmerge	0.090	0.790
Number of reflections	8931	586
<I/σ(I)>	27.61
Completeness [%]	96.4
Redundancy	19.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100mM Tris pH 8.0 and 20% w/v PEG 4000