7VP2

Structure of a transcription factor and DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-16
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	C 1 2 1
Unit cell lengths	74.633, 76.761, 46.010
Unit cell angles	90.00, 103.58, 90.00

Refinement procedure

Resolution	44.720 - 1.920
Rwork	0.180
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vp1
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.723	1.989
High resolution limit [Å]	1.920	1.920
Rmerge	0.103	0.434
Rmeas	0.112	0.482
Rpim	0.043	0.206
Number of reflections	19285	1888
<I/σ(I)>	13.65
Completeness [%]	99.8
Redundancy	6.2
CC(1/2)	0.996	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% (w/v) PEG 1000, 10% (w/v) PEG 8000