7VOK

The Crystal structure of EF-Tu and GDP from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-07-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	95.059, 75.180, 127.176
Unit cell angles	90.00, 93.77, 90.00

Refinement procedure

Resolution	29.950 - 3.400
R-factor	0.2473
Rwork	0.244
R-free	0.30160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1efc
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.520
High resolution limit [Å]	3.400	7.290	3.400
Rmerge	0.094	0.067	0.317
Rmeas	0.104	0.073	0.378
Rpim	0.043	0.028	0.201
Total number of observations	109018
Number of reflections	23713	2509	2241
<I/σ(I)>	7.9
Completeness [%]	94.9	96.6	90.7
Redundancy	4.6	6.4	2.9
CC(1/2)		0.994	0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	26% PEG 6000, 100mM N-2-hydroxyethylpiperazine-N-ethane-sulphonicacid pH 7.0