7VNU

Crystal structure of the N-terminal domain of SARS-CoV-2 nucleocapsid protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	P 21 21 21
Unit cell lengths	58.711, 91.412, 96.122
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.570 - 1.950
R-factor	0.1903
Rwork	0.188
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ofz
RMSD bond length	0.010
RMSD bond angle	1.597
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.570	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.050	0.698
Rpim	0.032	0.433
Number of reflections	38346	2633
<I/σ(I)>	19.8	2.6
Completeness [%]	99.8
Redundancy	6.5
CC(1/2)	0.999	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2M Sodium acetate trihydrate,, 0.1M sodium cacodylate pH 7.5, 4% PEG 8000