7VNS

Sandercyanin mutant E79A-Biliverdin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9897
Spacegroup name	P 63 2 2
Unit cell lengths	160.024, 160.024, 82.485
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.380 - 1.950
R-factor	0.1743
Rwork	0.174
R-free	0.17720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ez2
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.060	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	45772	3164
<I/σ(I)>	14.3
Completeness [%]	100.0	99.5
Redundancy	38.5
CC(1/2)	0.999	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.4 M sodium potassium tartrate, 0.2M HEPES pH 7.5