7VNO

Structure of aminotransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	113.378, 113.378, 290.041
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.990 - 1.800
R-factor	0.1745
Rwork	0.173
R-free	0.20220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2eo5
RMSD bond length	0.012
RMSD bond angle	1.685
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.133	0.092	0.713
Rmeas	0.138	0.095	0.740
Rpim	0.035	0.023	0.189
Total number of observations	1426489
Number of reflections	102595	5664	5036
<I/σ(I)>	5.3
Completeness [%]	100.0	99.9	100
Redundancy	13.9	16.7	12.8
CC(1/2)		0.996	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	PEG 3000, MgCl2, cacodylate