7VNL

Sandercyanin mutant-F55A-Biliverdin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9897
Spacegroup name	P 63 2 2
Unit cell lengths	158.388, 158.388, 81.073
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.360 - 1.930
R-factor	0.1949
Rwork	0.193
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ez2
RMSD bond length	0.008
Data reduction software	DIALS
Data scaling software	SCALA (3.3.22)
Phasing software	PHENIX (1.15.2-3472)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.120	2.030
High resolution limit [Å]	1.930	1.930
Rmeas	0.229
Rpim	0.026	0.581
Number of reflections	45351	6479
<I/σ(I)>	20.8
Completeness [%]	100.0	99.8
Redundancy	74.4	74.8
CC(1/2)	1.000	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1M sodium malonate pH 7, 12% PEG w/v 3350