7VN5

Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with double-stranded DNA contaning TTCACGTGAA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-12-07
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	102.622, 93.923, 112.750
Unit cell angles	90.00, 100.65, 90.00

Refinement procedure

Resolution	35.820 - 1.950
R-factor	0.2108
Rwork	0.209
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zd4
RMSD bond length	0.007
RMSD bond angle	0.835
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.960	1.980
High resolution limit [Å]	1.950	1.950
Number of reflections	152246	152246
<I/σ(I)>	3.4	3.5
Completeness [%]	99.4
Redundancy	3.4
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50 mM sodium cacodylate pH 6.5, 200 mM ammonium acetate and 10 mM calcium chloride and 10%