7VN3

Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with double-stranded DNA contaning CACACGTGTG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-12-19
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	102.656, 92.905, 111.935
Unit cell angles	90.00, 100.33, 90.00

Refinement procedure

Resolution	35.500 - 1.940
R-factor	0.2032
Rwork	0.202
R-free	0.23070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zd4
RMSD bond length	0.005
RMSD bond angle	0.653
Data reduction software	XDS (1.18_3855)
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.370	1.970
High resolution limit [Å]	1.940	1.940
Number of reflections	152181	7525
<I/σ(I)>	11.1	1.5
Completeness [%]	99.6
Redundancy	3.4
CC(1/2)	0.999	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	50 mM sodium cacodylate pH 6.5, 200 mM ammonium acetate and 10 mM calcium chloride and 10% (w/v) polyethylene glycol (PEG) 4000