7VMX

The Crystal Structure of EF-Tu and EF-Ts complex from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-05-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	128.114, 128.114, 200.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.860 - 2.800
R-factor	0.2334
Rwork	0.232
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1efc
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.900
High resolution limit [Å]	2.800	6.020	2.800
Rmerge	0.141	0.065	0.535
Rmeas	0.145	0.066	0.573
Rpim	0.036	0.015	0.191
Total number of observations	557995
Number of reflections	41642	4493	3763
<I/σ(I)>	5
Completeness [%]	98.9	99.7	91.2
Redundancy	13.4	18.4	6.2
CC(1/2)		0.998	0.250

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	100 mM TRIS hydrochloride pH 9.0, 8% PEG 6000, 2mM Zinc chloride