7VMW

Crystal structure of LimF prenyltransferase bound with a peptide substrate and GSPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	89.660, 48.430, 91.040
Unit cell angles	90.00, 117.87, 90.00

Refinement procedure

Resolution	40.240 - 1.930
R-factor	0.1843
Rwork	0.182
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tty
RMSD bond length	0.013
RMSD bond angle	1.228
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.240	2.000
High resolution limit [Å]	1.930	1.930
Number of reflections	52412	5193
<I/σ(I)>	11.67
Completeness [%]	100.0
Redundancy	6.9
CC(1/2)	0.998	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	PEG 1500, PCB (Na propionate, Na cacodylate, bis-tris propane) buffer, magnesium chloride