7VMT
Crystal structure of murine N-acetylglucosaminyl transferase IVa (GnT-IVa) lectin domain in unliganded form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2019-05-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 82.913, 82.499, 148.866 |
| Unit cell angles | 90.00, 105.66, 90.00 |
Refinement procedure
| Resolution | 46.963 - 1.950 |
| R-factor | 0.209566002051 |
| Rwork | 0.208 |
| R-free | 0.24413 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.932 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.740 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.065 | 0.629 |
| Rpim | 0.262 | |
| Number of reflections | 477251 | 67687 |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 6.8 | 6.7 |
| CC(1/2) | 0.999 | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Bis-tris (pH 6.5), 0.2M magnesium chloride, 25% (w/v) polyethylene glycol (PEG) 3350 |
