7VMI

Crystal structure of Arabidopsis thaliana HDT3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	103
Detector technology	CCD
Collection date	2019-09-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979470
Spacegroup name	P 1 21 1
Unit cell lengths	54.623, 77.600, 55.511
Unit cell angles	90.00, 91.82, 90.00

Refinement procedure

Resolution	35.260 - 1.800
R-factor	0.191
Rwork	0.190
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vmf
RMSD bond length	0.015
RMSD bond angle	1.759
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.130	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.037	0.495
Rmeas	0.051	0.678
Number of reflections	46503	2562
<I/σ(I)>	16.9	2.3
Completeness [%]	99.5	99.8
Redundancy	3.9	4
CC(1/2)	0.999	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M succinic acid pH 7.0, 0.1 M bicine, 30% V/V PEG MME 550