7VMI
Crystal structure of Arabidopsis thaliana HDT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2019-09-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979470 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.623, 77.600, 55.511 |
| Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
| Resolution | 35.260 - 1.800 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vmf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.759 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.130 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.037 | 0.495 |
| Rmeas | 0.051 | 0.678 |
| Number of reflections | 46503 | 2562 |
| <I/σ(I)> | 16.9 | 2.3 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.9 | 4 |
| CC(1/2) | 0.999 | 0.869 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M succinic acid pH 7.0, 0.1 M bicine, 30% V/V PEG MME 550 |
