7VMC

Crystal structure of EF-Tu/CdiA/CdiI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	101.930, 102.760, 88.140
Unit cell angles	90.00, 111.86, 90.00

Refinement procedure

Resolution	35.603 - 3.413
R-factor	0.2386
Rwork	0.237
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pc3
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.603	35.603	3.490
High resolution limit [Å]	3.400	15.200	3.400
Rmerge	0.308	0.077	2.437
Rmeas	0.328	0.084	2.631
Total number of observations	86262
Number of reflections	11523	134	719
<I/σ(I)>	7.12	19.5	0.85
Completeness [%]	98.1	91.8	84.8
Redundancy	7.486	5.582	6.321
CC(1/2)	0.995	0.995	0.324

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	22% PEG 3350, 100mM HEPES-KOH pH7.5, 2% Tacsimate pH7.0