7VMA

The X-ray crystallographic structure of amylo-alpha-1,6-glucosidase from Thermococcus gammatolerans STB12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	I 41
Unit cell lengths	108.087, 108.087, 225.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.998 - 2.804
R-factor	0.2483
Rwork	0.244
R-free	0.32800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	predict using alpha-fold
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.14_3260)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.770	2.600
High resolution limit [Å]	2.520	2.520
Rmerge	0.087	0.022
Number of reflections	44230	4569
<I/σ(I)>	12.3
Completeness [%]	99.8
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	25%tertiary butanol; 0.1M Tris